Clinicopathological characteristics and surgical treatment of duodenal gastrointestinal stromal tumor / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigate the clinicopathological characteristics and surgical treatment of duodenal gastrointestinal stromal tumor (GIST).</p><p><b>METHODS</b>Clinicopathological data of 25 cases with duodenal GIST from January 2007 to July 2011 in West China hospital were retrospectively analyzed.</p><p><b>RESULTS</b>All the patients were identified by pathological examination without specific symptoms. Tumors were located in the bulb area in 2 cases, descending portion in 11 cases, transverse portion in 8 cases, and ascending portion in 4 cases. Two cases were at very low risk, 7 at low risk, 6 at intermediate risk, and 10 at high risk. All the patients received surgical resection, including 11 pancreaticoduodenectomies, 10 local tumor resections, 2 duodenal segmental resections, and 2 distal subtotal gastrectomies. Eighteen patients were followed up from 16 to 39 months and 3 patients recurred 18, 30, and 35 months after operation respectively.</p><p><b>CONCLUSIONS</b>Duodenal GIST exhibits no distinct clinical characteristics. Complete removal of the tumor is the main choice of treatment.</p>

Single nucleotide polymorphism in flanking region of miR-30c influences the maturing process of miR-30c in lung carcinoma / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the effect of a common polymorphism rs928508(A/G) in flanking region of miR-30c on the expression of pri, pre and mature miR-30c, and discuss the effect of this polymorphism on the maturing process of miR-30c in lung carcinoma.</p><p><b>METHODS</b>The pGL3-promoter-miR-30c-A and pGL3-promoter-miR-30c-G luciferase plasmids were created containing A or G allele of miR-30c flanking region. Taqman assay was used to genotype rs928508 polymorphism in 50 lung cancer tissues. RT-PCR was performed to determine the expression of pri-miR-30c, pre-miR-30c, mature miR-30c and miR-30c host gene NFYC in the 50 lung cancer tissues.</p><p><b>RESULTS</b>The luciferase expression level of the pGL3-promoter-miR-30c-A construct group was not significantly different compared with that in the the pGL3-promoter-miR-30c-G construct group (A549 cells, P = 0.758; 293A cells, P = 0.554; CHO cells, P = 0.175). The results demonstrated that rs928508(A/G) variant had no effect on the transcriptional regulation of pri-miR-30c. In the genotype-phenotype collection analysis of the 50 lung cancer tissues, the expression of pre-miR-30c and mature miR-30c for rs928508 AG/GG genotypes showed significantly lower levels compared with those in the AA genotype (P = 0.009, P = 0.011). However, the expression of pri-miR-30c showed no significant difference between AG/GG genotypes and AA genotype. Similarly, the expression of host NFYC gene was correlated with pri-miR-30c, showed no significant difference between AG/GG genotypes and AA genotype.</p><p><b>CONCLUSION</b>The rs928508(A/G) polymorphism in flanking region of miR-30c could influence the processing from pri-miR-30c to mature miR-30c, but does not influence the transcription of pri-miR-30c.</p>

Effect of Skp2 antisense oligodeoxynucleotide on growth and proliferation of gastric carcinoma SGC-7901 cells / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the effect of S-phase kinase-associated protein 2 antisense oligodeoxynucleotide (Skp2 ASODN) on the growth and proliferation of gastric carcinoma SGC-7901 cells and its mechanism.</p><p><b>METHODS</b>The Skp2 oligodeoxynucleotides (ODNs) were embedded in cationic liposome Lipofectamine 2000 reagent and transfected into SGC-7901 cells. The cell growth and proliferation were observed with light microscopy and MTT assay. Cell cycle was measured by flow cytometry. The expression levels of Skp2 and p27 mRNA were detected by reverse transcription-polymerase chain reaction. The expression levels of Skp2 protein and its substrate p27 protein were detected by Western blot.</p><p><b>RESULT</b>After treatment with Skp2 ASODN, the growth and proliferation of SGC-7901 cells were inhibited in a dose-dependent manner with a peak value at 48 h. The inhibition rate of 200 nmol/L group at 48 h was 42.4 % (P<0.01). In cell cycle study the percentage of S phase cells in 200 nmol/L group was significantly higher than that in normal control group (P<0.05). Both Skp2 mRNA and its protein levels in 200 nmol/L group were significantly lower than those in control group and in Skp2 nonsense oligodeoxynucleotide (Skp2 NSODN) group (P<0.05). However, p27 mRNA level remained unchanged although its protein level was significantly higher than that in control group and NSODN group (P<0.05).</p><p><b>CONCLUSION</b>Skp2 ASODN can inhibit the growth and proliferation of SGC-7901 cells, which may be mediated by interfering with ubiquitin-proteosome pathway and cell cycle regulation.</p>

Expression of P53 and Bax in rat liver induced by microcystin LR in vivo / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To examine the expression of P53, Bcl-2 and Bax proteins in rat liver after exposed to microcystin LR.</p><p><b>METHODS</b>SD rats received microsystin LR by gastric perfusion. The expression of P53, Bax and Bcl-2 in liver was detected by Western blot.</p><p><b>RESULTS</b>The expression of P53 and Bax in each treatment group increased significantly compared with that in control group(P<0.05), with the exception of 0.1 microg/kg LR exposure group. Moreover, with exposure levels increasing the expression of P53 and Bax increased gradually; while no changes of the expression of Bcl-2 were observed.</p><p><b>CONCLUSION</b>P53 and Bax may play important roles in microcystin LR induced apoptosis, but Bcl-2 seems not be involved in this process.</p>